Human transformation of the global environment has initiated the sixth major extinction event in the history of life with the impact of global climate change already evident through range shifts, advanced phenology, and genetic change in natural populations. In fact, climate change is expected to exacerbate extinction threats for many species that are already threatened by habitat loss, fragmentation, and degradation. This interconnectedness – or the ability to move among habitat patches – is a primary component of long term population health and thus species persistence. By integrating population genetic and genomic approaches with measures of physiological stress, microclimate data, and non-invasive genetic mark-recapture efforts, it becomes possible to better understand and predict how wildlife species will respond to environmental change.